FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19910360
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA30550.022.3605921
AAAGTGC320750.018.359434
AGTAGCT198550.018.111275
CGAGTAG58550.018.0919693
CGTCGAG41800.017.3430481
GACTTGT167850.016.400043
CCAAAGT378800.016.392272
TAGCTGG253400.016.2266047
AAGTGCT373450.016.0485255
ACTTGTG188300.015.5812014
CAAAGTG398250.015.5566173
TGATCAA177450.015.5134639
GAGTAGC147350.015.2764174
CCGAGTA72900.015.0087132
CCCAAAG490200.014.7602061
GCTGGGA710950.014.6355299
GTCGAGA50800.014.5416422
GTGATCA195350.014.4487178
CTTGTGA212450.013.9412375
GTAGCTG261300.013.7886046
Produced by FastQC (version 0.11.5)