FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20069139
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG59500.020.145183
CGTCGAG41500.018.8134821
AGTAGCT209700.017.6796175
AAAGTGC324400.017.3790594
GACTTGT168200.017.2770563
CCGAGTA73900.017.2573412
TGATCAA175650.016.6241979
TAGCTGG254650.015.8455577
GTCGAGA50750.015.65438652
ACTTGTG195550.015.6089924
CCAAAGT381850.015.5676652
GAGTAGC150650.015.4040054
AAGTGCT371150.015.3777965
CGAGACT108900.015.2352071
CAAAGTG401650.014.8867243
GTGATCA199150.014.6975118
CTACTCG57750.014.4809322
CTTGTGA211550.014.4284855
GCTGGGA712400.014.2531379
TTGTGAT214550.013.96688656
Produced by FastQC (version 0.11.5)