FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18449369
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC318200.022.3805054
CGAGTAG56550.021.7812523
AGTAGCT209400.021.5006965
CCAAAGT370650.020.2444922
AAGTGCT364400.019.717865
CCGAGTA64350.019.3610612
CAAAGTG387500.018.8709953
TAGCTGG250050.018.7699157
GAGTAGC146650.018.1983224
GCTGGGA689100.018.049459
CCCAAAG473600.017.9064271
GTAGCTG253650.017.6102926
AGTGCTG465600.015.6145536
CTACTCG57050.014.8898612
GCTACTC146450.014.3559161
CCCGAGT123200.013.560171
GTCGAGA39350.013.49212551
AAGTAGC131650.013.4428454
TACTCGG65300.013.3339283
CGAGACT93450.013.1805571
Produced by FastQC (version 0.11.5)