FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18449369
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC322200.022.0252764
CGAGTAG59700.021.4495033
CTACTCG55050.020.832452
AGTAGCT201650.020.4243035
CCAAAGT374750.020.2127232
AAGTGCT365850.020.1165185
CCGAGTA66550.020.1046472
TAGCTGG251600.019.0707387
CAAAGTG388700.018.8745773
GAGTAGC143400.018.0090054
GCTGGGA706100.017.8612219
CCCAAAG483000.017.6943341
TACTCGG64000.017.4658663
GCTACTC146400.017.135481
GTAGCTG252400.016.8226746
AGTGCTG460900.016.5825046
CCCGAGT121100.013.9858171
GCTACTT130900.013.3177111
GCTAATT168750.013.1862091
GTGCTGG635800.012.8449887
Produced by FastQC (version 0.11.5)