FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18099140
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC313050.022.5442964
CGAGTAG56750.022.0755253
CCAAAGT359450.021.1972872
AAGTGCT354600.019.7238945
AGTAGCT194600.019.534665
CAAAGTG384000.019.3351673
CCGAGTA64850.019.3272382
CCCAAAG462800.018.3042981
TAGCTGG242350.018.2869197
GCTGGGA674450.018.1915659
CTACTCG56050.017.308182
GAGTAGC140800.017.2946194
AGTGCTG451950.016.244046
GTAGCTG244100.016.1834876
GCTACTC142950.015.6704491
TACTCGG68650.014.6403483
GCTACTT126450.013.17431
CTACTTG127200.013.0267252
CCCGAGT115050.012.7544651
GTGCTGG614150.012.5073287
Produced by FastQC (version 0.11.5)