FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18448341
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG57850.022.6419123
AAAGTGC318850.020.7400554
CCGAGTA69400.019.89392
AGTAGCT209100.019.5375465
CCAAAGT370200.019.4695592
AAGTGCT364350.018.4997755
TAGCTGG253600.018.0911357
CAAAGTG390000.017.8275493
CCCAAAG475600.017.3021811
GAGTAGC148700.017.0459634
GTCGAGA39350.016.9168571
GCTGGGA699400.016.8140939
CTACTCG57050.016.5059592
GTAGCTG261400.015.6827166
CCCGAGT122300.014.881381
AGTGCTG467250.014.8651066
TACTCGG65350.014.3012213
CGAGACT94850.013.513811
GCTACTC148700.013.4299591
GCTATAT69400.013.1633621
Produced by FastQC (version 0.11.5)