FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20131530
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC310100.019.3076784
CCAAAGT364600.017.9196762
AAGTGCT359100.017.0091045
CAAAGTG378050.016.8880883
CGAGTAG59950.015.8563353
AGTAGCT196500.015.66826255
CCCAAAG496950.015.2030831
TAGCTGG242150.014.3550687
GCTGGGA726950.014.2085749
CCGAGTA71950.013.8033482
CTACTCG57000.013.4411392
GAGTAGC144950.013.4097814
GCTACTC138150.013.2997551
AGTGCTG478150.013.0412246
ACGTCGA32150.012.7979911
GCTAATT151200.012.5272051
CGTCGAG42550.012.3374931
GTAGCTG261950.011.61803156
GACTTGT154900.011.5408113
GTCGAGA49950.011.5036782
Produced by FastQC (version 0.11.5)