FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20081291
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC312100.018.471154
CCAAAGT365500.017.0344052
AAGTGCT365100.015.8480755
CAAAGTG388850.015.7371023
AGTAGCT198500.015.6820295
CGAGTAG57000.015.6744313
TAGCTGG243750.015.1559337
CTACTCG55800.014.8686492
CCCAAAG499200.014.4498581
GCTGGGA729850.014.4462469
CCGAGTA69650.013.8464232
GCTAATT152050.013.4812021
GAGTAGC145650.013.3394284
GCTACTC143650.013.3313871
GTAGCTG265300.012.5352746
AGTGCTG491050.012.3752316
GCTATAT61000.012.0926281
TACTCGG69950.011.961633
CGTCGAG40400.011.9383621
GCTACTT137150.011.8428671
Produced by FastQC (version 0.11.5)