FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19646153
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC298800.019.5581054
CGAGTAG53000.018.531673
CCAAAGT355650.017.6749692
AAGTGCT347650.017.2539025
CTACTCG49150.017.1289652
CAAAGTG371400.016.2638593
AGTAGCT185350.015.8971845
CCCAAAG465550.015.4169411
TAGCTGG225950.015.2724617
GCTGGGA648300.015.2438069
CCGAGTA65400.014.9111272
TACTCGG59650.014.3486743
GCTACTC133950.014.1943481
GAGTAGC133550.014.1309314
AGTGCTG452450.013.5366266
CGTCGAG35950.013.4660161
GCTAATT144400.012.7784291
GTAGCTG239850.012.6945356
GACTTGT156350.012.6087143
CCTATAT69450.012.5267481
Produced by FastQC (version 0.11.5)