FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19050312
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC283300.019.8408644
CCAAAGT338050.018.1268772
CAAAGTG354400.016.8678873
AAGTGCT340500.016.5905555
CCCAAAG444850.016.1006811
CGAGTAG49050.015.7166183
GCTGGGA628300.015.0055889
CCGAGTA60050.014.2442392
AGTAGCT176400.014.2241885
TAGCTGG213050.013.8837957
AGTGCTG438150.013.4532276
CTACTCG49500.012.4643572
GAGTAGC130700.012.4407094
GCTAATT131050.012.0441891
GCTATAT64450.011.8641241
TACTCGG60450.011.5933533
GCTACTC144650.011.1057821
GTAGCTG233050.011.0675626
GCTACTT126050.011.0193121
TGCTGGG677400.010.9065818
Produced by FastQC (version 0.11.5)