FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19050312
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA27900.024.3881421
AAAGTGC291100.019.2262384
CGTCGAG38100.018.5931952
CGAGTAG51300.018.5931473
CCAAAGT341650.017.6963392
AAGTGCT339100.016.9391215
CTACTCG49300.016.9300272
CCGAGTA60700.016.408062
CAAAGTG363250.016.3341353
CCCAAAG448150.016.0909211
AGTAGCT178450.015.3279065
GCTGGGA624400.015.1078329
TAGCTGG214300.014.4981997
AGTGCTG438350.013.9358396
TACTCGG60750.013.738273
GTCGAGA50700.013.55656053
GACTTGT154550.013.3416553
GAGTAGC128850.012.9546654
GCTACTC135050.012.6218461
GCTAATT134900.012.3758811
Produced by FastQC (version 0.11.5)