FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20239225
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC305750.019.3827954
CCAAAGT360200.017.7400672
CGAGTAG53200.017.6863823
AAGTGCT364500.016.5282125
AGTAGCT192050.016.5257155
CAAAGTG383050.016.0764433
TAGCTGG231400.015.6548737
CCGAGTA63950.015.5921282
CCCAAAG479950.015.583651
GCTGGGA668250.015.1282569
GAGTAGC140000.014.2941164
AGTGCTG468750.013.7809946
GCTAATT152650.013.5719031
CCTATAT71250.013.10938551
GTAGCTG250600.012.8327116
GTCGAGA44250.012.6967421
GCTACTC133750.012.4967981
GACTTGT164500.011.7811853
TGATCAA167800.011.7147069
CGAGACT93500.011.2666621
Produced by FastQC (version 0.11.5)