FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19411706
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC292250.019.8444124
CCAAAGT349200.018.3345072
AAGTGCT337550.017.3902995
CAAAGTG370650.016.7990933
CCCAAAG458000.015.957361
GCTGGGA647750.015.0840989
CGAGTAG49700.014.5317433
CCTATAT69050.014.5255041
AGTAGCT178600.014.4113385
AGTGCTG454250.013.8339846
GCTAATT129950.013.547411
TAGCTGG222650.013.3881857
CCGAGTA60350.012.549292
GCTATAT62250.012.5066931
CTACTCG48150.012.3792782
GAGTAGC133200.011.7931824
GTGCTGG610100.011.3467537
GCTACTC149000.011.2975381
CTGTCTA136100.011.257737145
TGCTGGG701300.011.06154258
Produced by FastQC (version 0.11.5)