FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19646153
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC297200.019.1037224
CCAAAGT356650.017.2844332
CGAGTAG50000.016.8227673
AAGTGCT352500.016.3464835
CAAAGTG373950.016.1952023
CTACTCG52400.015.7928032
CCCAAAG462100.015.7178411
GCTGGGA647600.015.0158089
AGTAGCT182150.014.6239965
CCGAGTA59050.014.6080992
TAGCTGG219650.013.7561347
GAGTAGC130000.013.3718074
AGTGCTG462150.013.2879576
TACTCGG63600.013.1152333
GCTAATT140050.012.97792151
CTGTCTA141050.012.215281145
GCTACTC147250.012.2003751
GCTATAT62150.011.7430451
GTAGCTG241550.011.1445176
GTGCTGG625650.010.9026417
Produced by FastQC (version 0.11.5)