FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19411706
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC294150.019.395534
ACGTCGA28650.018.3752381
CCAAAGT342600.018.0878472
CGTCGAG39950.017.921721
AAGTGCT345250.016.7077985
CAAAGTG363850.016.0850123
AGTAGCT180150.015.8930415
CCCAAAG451900.015.4863531
TAGCTGG219500.015.4094017
CGAGTAG53400.015.2438433
GCTGGGA646550.015.2059569
GTCGAGA49800.014.656072
CCGAGTA63500.013.7044482
GAGTAGC135200.013.4951174
AGTGCTG449900.013.4921176
CTACTCG48900.013.0600872
GCTACTC136550.012.8512281
GTAGCTG236000.012.5779056
GACTTGT157900.012.1327233
CCTATAT68350.011.9128541
Produced by FastQC (version 0.11.5)