FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19745816
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC304350.019.0134074
CCAAAGT353650.018.4022062
CAAAGTG376050.016.7092573
AAGTGCT353650.016.484115
CCCAAAG462450.015.9487061
AGTAGCT190300.015.4273425
GCTGGGA670050.015.0212919
TAGCTGG233050.014.4050787
CGAGTAG53750.013.814583
GCTAATT151550.013.6471061
AGTGCTG456400.013.45096
GTAGCTG248700.012.2576266
CCTATAT70900.012.1627951
GAGTAGC135850.012.0157114
CCGAGTA65550.011.8625542
CTACTCG54000.011.8052592
GCTACTC145400.011.1865991
GTGCTGG632550.011.0133837
GCTATAT68300.010.57282451
TACTCGG64550.010.3095023
Produced by FastQC (version 0.11.5)