FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19483725
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC295150.019.9303534
CCAAAGT355550.018.080292
AAGTGCT345700.017.3629135
CGAGTAG56750.017.1716213
CAAAGTG374850.016.6641253
CCCAAAG462350.015.7802831
TAGCTGG230250.015.6254617
AGTAGCT192100.015.5865035
GCTGGGA661000.015.3118999
CCGAGTA65800.014.9155362
GAGTAGC137650.014.3128434
CTACTCG53150.013.8491382
AGTGCTG448600.013.5996676
GCTAATT145500.013.4522061
GTAGCTG246100.012.8223966
GTGCTGG613200.010.9681047
CCCGAGT122800.010.9115711
GCTACTC136050.010.8801821
TACTCGG66400.010.8750563
GCTATAT63600.010.6995841
Produced by FastQC (version 0.11.5)