FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19635852
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC307400.018.1027014
CCAAAGT355350.017.1213932
AGTAGCT200400.016.6329775
CGAGTAG59900.016.5182953
AAGTGCT355000.016.2881935
GACTTGT172100.015.8206313
CAAAGTG374650.015.7148113
TGATCAA174850.015.6521159
CGTCGAG41450.015.5806491
ACGTCGA27750.015.4308231
TAGCTGG244050.015.1820457
CCCAAAG462300.015.0781341
ACTTGTG194700.014.6330254
GCTGGGA665800.014.5764729
GTGATCA193850.014.1903898
GTCGAGA50950.014.0532981
CCGAGTA70000.014.03480152
GAGTAGC149700.013.9691034
CTACTCG53400.013.7982612
CGAGACT97400.013.6935771
Produced by FastQC (version 0.11.5)