FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18338098
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA26550.019.954961
AAAGTGC286950.018.4124
CGAGTAG52550.017.8764363
CGTCGAG36550.017.7809331
CCAAAGT338000.016.5512892
AAGTGCT334050.015.9856425
AGTAGCT177550.015.8734755
CAAAGTG355950.015.4975853
GTCGAGA46150.014.9995332
CCGAGTA64650.014.6406242
CCCAAAG441950.014.625211
GCTGGGA644200.014.4960429
TAGCTGG225200.014.1463157
GAGTAGC131100.013.7924964
GCTAATT136850.013.5241691
CGAGACT93200.012.6576971
AGTGCTG441650.012.4912076
GTAGCTG236200.012.08184056
CTACTCG51600.011.361922
CCCGAGT117300.011.2615391
Produced by FastQC (version 0.11.5)