FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18647757
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC289900.018.2845234
CCAAAGT350700.016.6300032
AAGTGCT338650.016.0073095
CGAGTAG51350.015.5354643
CAAAGTG369400.015.2507873
AGTAGCT185500.015.1442365
CCCAAAG461350.015.1135521
CTACTCG50550.015.0851832
GCTGGGA652250.014.4195649
CCGAGTA59400.014.2640492
TAGCTGG221100.013.8589137
TACTCGG61350.013.118273
GCTAATT135850.012.7404981
AGTGCTG447300.012.6591586
GCTACTC130800.012.5302621
GAGTAGC132600.012.0829854
GTAGCTG243950.011.866226
GCTACTT123350.011.5689031
GCTATAT58500.010.8684231
CTACTTG121100.010.4365482
Produced by FastQC (version 0.11.5)