FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16230845
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC245050.018.430324
CCAAAGT287900.017.3963222
CGAGTAG45800.017.048233
AAGTGCT283750.016.339535
AGTAGCT154800.015.9524055
CAAAGTG306300.015.8920833
CCGAGTA54600.015.5887692
TAGCTGG184950.015.0659037
CCCAAAG380350.014.9994011
GCTGGGA537250.014.5650099
CTACTCG44850.014.1155942
GAGTAGC116100.013.6939514
GCTAATT113750.013.60528851
GTAGCTG204850.012.7765346
AGTGCTG379200.012.70949656
CTGTCTA116800.012.298633145
GCTACTC112150.011.9803791
TACTCGG53850.011.364633
CCCGAGT100650.011.1127061
GCTACTT108050.010.4818411
Produced by FastQC (version 0.11.5)