FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16025730
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC247250.018.5499674
CCAAAGT282000.017.8832452
AAGTGCT289650.016.3697195
CAAAGTG301850.016.2892193
CCCAAAG379650.015.6022161
GCTGGGA536550.014.6096939
AGTAGCT153050.014.2949295
CTACTCG41600.014.2024152
TAGCTGG186500.013.9964027
CGAGTAG41100.013.5207093
AGTGCTG374800.012.9521656
GAGTAGC110550.012.9172224
CCGAGTA48250.011.9534372
GTAGCTG199450.011.7110796
GCTAATT111900.011.6305231
CTGTCTA113300.011.539491145
TACTCGG54000.011.0723393
GCTACTC111500.010.9782221
GCTATTA59200.010.9326071
CCTATAT57900.010.8135691
Produced by FastQC (version 0.11.5)