FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16154235
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG44950.017.5578783
AAAGTGC245100.017.1057684
CCAAAGT288650.016.1614722
AGTAGCT156450.015.2683835
AAGTGCT286350.015.2075835
CCCAAAG375300.014.7213661
CCGAGTA51950.014.6498342
CAAAGTG300050.014.6311393
TAGCTGG186700.014.60555657
GCTAATT122400.014.5663161
GAGTAGC112300.014.5571784
GCTGGGA539800.014.345529
CTACTCG44650.014.0463342
AGTGCTG373500.012.4132866
CGAGACT72500.012.344581
TACTCGG54350.012.0577743
GTAGCTG198500.011.9979676
GCTACTC114350.011.8324771
GCTATAT52750.011.7563041
GACTTGT124450.011.7208513
Produced by FastQC (version 0.11.5)