FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16154235
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC245000.018.7172934
CCAAAGT292100.016.903882
AAGTGCT292700.016.0043435
CAAAGTG307000.015.8088253
CCCAAAG385350.014.9344581
GCTGGGA532250.014.348769
AGTAGCT151750.014.2975275
CGAGTAG43150.014.202253
TAGCTGG180950.013.8210897
AGTGCTG377550.012.9865916
GCTAATT110400.012.888581
CTACTCG42650.012.8817682
GAGTAGC112900.012.2289814
GCTACTC107150.011.8989641
CCGAGTA51950.011.7959682
GTAGCTG200550.011.5566956
TACTCGG51850.010.7324053
TGCTGGG577850.010.6310618
GTGCTGG506150.010.6198367
CTGTCTA116200.010.440053145
Produced by FastQC (version 0.11.5)