FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21292835
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC323500.018.7473684
CGAGTAG59800.017.8339393
CCAAAGT381950.017.5966262
AGTAGCT204850.016.7094755
AAGTGCT379400.016.0748625
CAAAGTG402300.015.8380463
GCTACTC154700.015.1299241
GCTGGGA732800.015.0890429
CCCAAAG512750.014.8753981
TAGCTGG258550.014.8414817
CCGAGTA71300.014.6781332
CTACTCG60450.014.6145912
GAGTAGC155000.013.6705514
CTGTCTA139700.012.999502145
GTAGCTG266550.012.867476
TACTCGG75500.012.6858913
AGTGCTG506300.012.6632326
GCTATAT62600.012.4994851
GCTAATT158600.012.31253051
CCTATAT65300.011.0448881
Produced by FastQC (version 0.11.5)