FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20920613
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG60300.018.4710523
AAAGTGC324100.018.2726944
CCAAAGT378200.017.4012662
AGTAGCT204550.017.0991655
AAGTGCT374300.016.2212725
CCGAGTA74700.015.8200282
CAAAGTG408400.015.6483663
TAGCTGG246200.015.2272457
CTACTCG59150.015.1564612
GCTGGGA726450.014.9183099
CCCAAAG508900.014.89578151
GAGTAGC149750.014.4219964
GCTAATT157600.013.8350381
GACTTGT153150.013.7914053
GTAGCTG267500.013.1516556
TGATCAA155350.012.8507519
GCTACTC150000.012.815241
ACTTGTG174050.012.4865094
AGTGCTG498900.012.4833256
CGAGACT93850.011.8697381
Produced by FastQC (version 0.11.5)