FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20927042
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC327050.017.7277074
AGTAGCT199400.017.657645
CCAAAGT379200.016.863782
CGAGTAG62550.016.5163963
AAGTGCT369250.016.1990765
CAAAGTG395600.015.6001433
CCGAGTA73500.015.5330092
TAGCTGG244750.015.4419427
CTACTCG61050.015.3612672
GAGTAGC154800.015.1044594
CCCAAAG505750.014.3925941
GCTGGGA718550.014.3131399
GTCGAGA46200.014.2697131
GTAGCTG259450.013.5448856
GCTACTC151300.013.3414511
TACTCGG72950.012.671053
GCTAATT161500.012.6250831
CGAGACT100200.012.6162621
AGTGCTG493150.012.4740656
CCTATAT61900.012.2973881
Produced by FastQC (version 0.11.5)