FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21159517
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC321600.019.9948654
CGAGTAG62000.018.9474283
CCAAAGT381100.018.5129032
AAGTGCT372200.017.6607115
AGTAGCT200550.017.3024435
CAAAGTG402600.016.8326553
CTACTCG60700.016.6684592
CCGAGTA74350.016.074212
CCCAAAG507850.015.72796251
GCTGGGA733200.015.5979249
TAGCTGG251700.015.1626687
GAGTAGC152050.014.60312754
GCTACTC152500.013.9849331
AGTGCTG501400.013.5025866
GTAGCTG269600.012.9212236
TACTCGG76300.012.8154493
CTGTCTA144950.012.439906145
CCCGAGT135000.012.175371
GCTAATT154900.012.0581831
GCTACTT137550.011.5052451
Produced by FastQC (version 0.11.5)