FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005472359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21159517
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC330750.017.7214434
AGTAGCT205550.017.677985
CCAAAGT384000.016.8898742
AAGTGCT374200.015.9725865
TAGCTGG258100.015.8686147
GTCGAGA43500.015.7708151
CAAAGTG406900.015.4729093
CGAGTAG61750.015.3982363
CTACTCG59900.014.7390942
GCTGGGA741000.014.4279089
GAGTAGC153800.014.2196094
CCCAAAG510600.014.1541181
GCTACTC151500.013.5847621
CCGAGTA74000.013.3073192
GTAGCTG277350.013.1506626
GACTTGT158400.012.9488653
TACTCGG72800.012.4079533
AGTGCTG498950.012.3194986
TGATCAA163550.012.1684139
ACTTGTG181600.012.1176194
Produced by FastQC (version 0.11.5)