FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005472680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005472680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316107997
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA6047256519.130349619089202No Hit
GCTCAACC5472529917.312215926002025No Hit
TGCGGCTT4629477314.645239424297133No Hit
CAAAGTGG4003314112.664387291663488ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CCACTACA3731801411.805463434700767No Hit
ATGAAGAC312058579.871897356649285No Hit
GATTCTGG296556029.381477938376865No Hit
TTGTTGAT164027465.188969009221238No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)