FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30181193
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC216850.025.00148613
GGGATTA236500.024.95162612
GATTACA227650.023.6616414
ATTACAG254900.020.69280615
TGGGATT318550.019.97502111
TTACAGG271650.019.09486816
TACAGGC247950.018.28016917
AAAGTGC249800.017.5283434
CCAAAGT282650.016.5685522
CTGGGAT425800.015.45336810
AAGTGCT289100.015.3393145
CAAAGTG303350.015.1033433
ACAGGCG176050.014.990334518
TGATCAA127750.014.6027169
GGCGTGA151550.014.57296721
GCGTGAG157300.013.92889222
CCCAAAG391700.013.9053881
AGTAGCT152550.013.9039395
AGGCGTG170300.013.74954120
GACTTGT139200.013.7036013
Produced by FastQC (version 0.11.5)