FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30181193
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346120.1146806887322181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC220350.025.21730413
GGGATTA245600.024.49104312
GATTACA236850.023.5197314
ATTACAG260200.021.3421115
TGGGATT332700.019.75128711
TTACAGG278300.019.33803216
TACAGGC257150.018.57454317
AAAGTGC246600.016.9695034
CCAAAGT282650.016.11172
ACAGGCG178650.015.31689518
ATCGCTT41750.015.0861127
CTGGGAT433850.015.06529510
CGAGTAG51000.014.9565843
CAAAGTG300350.014.6903983
AAGTGCT286200.014.6213275
AATCGCT37900.014.49521126
GGCGTGA153200.014.32110321
AGTAGCT160250.013.8643945
CCCAAAG390250.013.6957361
AGGCGTG168200.013.58471520
Produced by FastQC (version 0.11.5)