FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30665768
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC220400.024.0093713
GGGATTA238600.023.99680712
GATTACA235750.022.5795514
ATTACAG258700.020.31951715
TGGGATT318900.019.5018111
TTACAGG282050.018.57556216
TACAGGC253750.017.43343717
AAAGTGC249150.016.6995814
CCAAAGT286000.016.0118832
ACAGGCG177050.015.14163418
CTGGGAT420900.015.09168110
AAGTGCT289750.014.6618845
CAAAGTG298050.014.5826663
GGCGTGA154650.014.00951621
CCCAAAG392600.013.786721
GCGTGAG160350.013.55554722
CGGCTAA34700.013.51550239
AGTAGCT158450.013.4278335
CGTGAGC162900.013.2790223
AGGCGTG171550.013.26187720
Produced by FastQC (version 0.11.5)