FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28919497
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1374880.4754162909541615No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC548560.1896851802090472No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG470630.16273796186704076No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA321580.11119833792406555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC60850.028.330192
GGTGGCG224300.025.3347871
GCGATCT70550.024.521483
GGGCGAT74700.024.1624641
TGGCGCG193850.023.4936893
CGGTGGC136150.022.529181
GGCGCGT219250.020.3750364
CGATCTG87850.020.0080974
GTGGCGC292100.019.3471682
GCGCGTG243100.018.5179585
CGGTAGT31900.018.51086613
CGCGTGC251250.017.695866
GCCACGA137100.017.00044619
AGTGCGT136050.016.62248630
GCGTGCC279650.016.0599967
GAGTGCG143350.015.99404529
TAGCATA63650.015.7710719
CGTGCCT297200.014.8076138
CGATAGG13450.014.72279239
GCGTCCT154900.014.62220133
Produced by FastQC (version 0.11.5)