FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31192535
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1592150.5104266132906479No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT967540.310183189663809No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG908760.29133893734510513No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA831570.26659263185887266No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC797510.255673352614656No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT642020.20582488726870068No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT597070.19141438808997088No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC512150.16418992557033277No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA481210.1542708856461971No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG425200.13631466631359074No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT398200.12765874912058287No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA392970.12598206590134467No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC354230.1135624276770067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG427500.040.08819270
ACCGCGT39900.029.3665496
CCGCGTT40800.029.1422397
CCCGATC449650.028.5748469
AAATGCG60800.026.28883770
ATACGGA46450.025.74829533
TACGGAG46950.025.6226434
AGACCGC46400.025.5522334
GTGCGAT122500.025.2616081
GCGATCG125550.024.8130573
AATACGG51050.024.58556732
CAATACG48300.024.54569631
ACCCGAT486200.024.5076768
CGCGTTC48950.024.0771648
GAACGAT49650.023.74391245
CACCCGA504750.023.29061967
TACCGGC56500.023.22818670
CGATCGG378600.022.7274170
CCTACGT131200.022.70887425
TGCGATC136450.022.6524812
Produced by FastQC (version 0.11.5)