FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31192535
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG612440.19634184909947203No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC444590.14253089721627307No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG406800.13041581904131871No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT402120.12891546006119733No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCA325650.10439997903344501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC171250.026.6681231
GTCTAAC83250.025.402571
CATGCCG129250.022.95366320
ACGTGCG79400.022.8818198
TAACACG79900.022.8705274
CCGACGG132550.022.72360624
CGCGAGT95750.021.55086313
GCCGACG140600.021.39752623
TGGCGCG186450.020.981353
CGCACGA88150.020.49438928
GGTAATT151700.020.4750161
CTAATAC148250.020.40988214
GCTAATA150000.020.26440213
TGCGCGA101800.020.2356111
AACACGT92550.020.0065085
CTAGAGC156450.019.8282898
TGCCGAC154000.019.73843422
GGTGGCG239450.019.6390361
GTAATTC160400.019.5249632
CGAGTCG93900.019.45987115
Produced by FastQC (version 0.11.5)