FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478629
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2048860.6950323232467833No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG583010.19777378384863148No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA458160.15542106792008542No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA457880.155326083855528No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC437710.14848383891937444No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT346350.11749189556949885No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG336900.11428618339068618No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT336150.11403176178919311No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTCCCATGTCCCTTGGGAAGGTCTG298780.1013547814587985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC66300.031.5491832
GGGCGAT69750.030.7573681
GGTGGCG285600.027.5386471
GCGATCT79350.026.4816423
TGGCGCG294750.024.5572833
CGGTGGC159300.023.6822071
GGCGCGT311650.022.6449244
CGATCTG96550.022.2305664
CGGTAGT31600.021.65052413
GTGGCGC361200.021.6248822
GCGCGTG341400.020.7618055
CGCGTGC343350.020.3707266
GCGTGCC385500.018.350657
GCCACGA130300.017.64408519
CGTGCCT399100.017.44698
AGTGCGT137500.016.72097230
GAGTGCG138800.016.61409829
ATAGCGT16350.016.57698650
TCGCACG27100.016.40325227
TAGCGTG16950.016.40086651
Produced by FastQC (version 0.11.5)