FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25379643
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC185650.024.4269613
GGGATTA203800.023.77943812
GATTACA198650.022.61708814
ATTACAG214550.020.59863715
TGGGATT269850.019.68336311
TTACAGG231850.018.6541316
TACAGGC209200.017.74672517
AAAGTGC204850.016.9953544
CCAAAGT235250.016.3542042
ATCGCTT35400.015.42011427
CTGGGAT356250.015.2236310
CAAAGTG245750.015.0647463
AAGTGCT234700.015.01254755
AATCGCT33400.014.87675226
ACAGGCG149300.014.85909218
CGAGTAG44500.013.83956053
CCCAAAG325650.013.7676031
GCGTGAG133450.013.53017222
GGCGTGA132800.013.49089221
GAATCGC35650.013.34903625
Produced by FastQC (version 0.11.5)