FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31357331
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC215000.024.9885613
GGGATTA235400.024.47338312
GATTACA234300.023.04953614
ATTACAG259050.020.4691115
TGGGATT323050.019.79411111
TTACAGG275250.018.97195616
TACAGGC254650.017.97769517
AAAGTGC252550.016.5616514
CCAAAGT284550.015.5110432
CTGGGAT420700.015.17467810
ACAGGCG183800.014.70087318
CAAAGTG299600.014.6617473
ATCGCTT43750.014.56015727
AAGTGCT291300.014.50266555
GGCGTGA156750.013.97766621
GCGTGAG160550.013.82123522
AATCGCT40600.013.62079326
CGTGAGC164100.013.60750823
CCCAAAG398050.013.30555251
TGATCAA129950.013.2512049
Produced by FastQC (version 0.11.5)