FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29113422
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA223700.024.95567312
GGATTAC206000.024.94212313
GATTACA222200.023.34406714
ATTACAG244950.021.11895215
TGGGATT301600.020.1925811
TTACAGG260600.019.24629416
TACAGGC237000.018.37152317
AAAGTGC231000.017.3489864
CCAAAGT272050.015.9663032
CTGGGAT393600.015.75732510
ACAGGCG168100.015.09535818
AAGTGCT269600.015.0467465
GGCGTGA143400.014.69318721
CAAAGTG284100.014.6238193
CGTGAGC151700.014.05065623
GCGTGAG148950.014.00459922
TGATCAA122950.013.9773439
CAGGCGT158450.013.84993219
CGAGTAG51100.013.6305543
AGGCGTG164200.013.40756420
Produced by FastQC (version 0.11.5)