FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29113422
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA231250.025.22374712
GGATTAC212700.025.2028713
GATTACA226500.023.65196414
ATTACAG250750.021.3227215
TGGGATT307450.020.2351111
TTACAGG269950.019.19716816
TACAGGC237850.018.09552217
AAAGTGC237900.017.193164
CCAAAGT271500.016.3354242
CTGGGAT405100.015.4348510
ACAGGCG167450.015.33837418
CAAAGTG284600.015.2330793
AAGTGCT276750.014.8425285
GGCGTGA146150.014.60505421
GCGTGAG153600.013.73727122
CCCAAAG377400.013.7348231
CGTGAGC152900.013.731623
AGGCGTG165650.013.56158720
ATCGCTT39400.013.49960427
AGTAGCT156650.013.37900455
Produced by FastQC (version 0.11.5)