FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48945549
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTAG227050.018.1109281
GATGAAA122950.018.044621
GCGCAAC197750.017.966821
CAACTAG225450.017.9333271
CCGGGAT163000.017.8452051
TGCCCAT280250.017.8280811
ACTAGCA173350.017.803113
AGCGATA188450.017.8029428
GGGAGAT134000.017.7733521
GGGACCT186950.017.720651
CGCCAGA181850.017.6912462
CGGACAC167450.017.681591
GACTACA217500.017.6736351
CGGACGT139850.017.5949251
ACGAGCC148750.017.5844761
AAACGGG213400.017.506971
CGACCTT232550.017.4331171
CTAATGG182000.017.4276811
CTAAGAC129150.017.3642481
TCGAATG162450.017.3466938
Produced by FastQC (version 0.11.5)