FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005474697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005474697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29468295
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTTGTCTCTTATACACATCTCCG331340.11243948793101197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCATA74700.019.5196195
GCCCCAT77300.019.0180644
AAGAGGC241650.018.7088039
CTAAGAC132500.018.699121
CGACAAG210150.018.6928925
CCCATAA78250.018.6468456
ATAAGAC96350.018.5987879
CATACCA207650.018.5851883
AGCGACA222550.018.5272033
TACCATG198100.018.5224175
TATGCCC145100.018.4885311
ATACCAT201550.018.4680964
TGCCCAT132450.018.4645181
TTGAGAC117850.018.44676210
GCGACAA212550.018.396884
GCATACC209350.018.3433512
CACACAA205900.018.31941
GCCCATT95350.018.3123442
AGCATAC261600.018.2959631
AGGATGG113400.018.2804158
Produced by FastQC (version 0.11.5)