FastQCFastQC Report
Thu 23 May 2024
EGAF00005475266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563191106
Total Bases37.1 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC132375092.3504470967267017No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGG67412711.196977531814929No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA60997681.0830725015036016No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT56142040.9968559411163712No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT55068670.9777972239497689No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT47211430.8382843673671224No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC35011670.6216658897308652No Hit
GCATATAGAGGCAAGAAAGAGATACCAAGATGCCATCTGTGTCCTGGTTA34181910.6069327025203413No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG33318600.5916038027773826No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT32223770.5721640426615685No Hit
TAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGG31988270.567982513559083No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT30223060.5366395114911491No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC25488300.45256929181690597No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA22942320.4073629671275384No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCT21498930.3817341888208014No Hit
CAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGG20828640.3698325449052812No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA19326710.34316433257026613No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC17583510.3122121392307641No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT17540910.3114557352402508No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG16423430.29161380258018493No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA16399260.29118464097336083No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT15090590.2679479458967166No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG14588080.25902539732223684No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT13137990.2332776540686351No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT12605650.2238254451411738No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC11800910.20953651210535987No Hit
TAGGTGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAAT11021220.19569236592312236No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA9949620.17666507680964694No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT9936370.17642981031025018No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA9850240.17490048928436025No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC9112560.16180227107492712No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG8795330.1561695471803136No Hit
AGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATA8516500.1512186522348952No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA7892240.14013431526029818No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG7152960.1270076875113152No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT6534240.11602171856740934No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA6354470.11282972923936764No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG6311800.11207208233149904No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG6222520.11048683002462045No Hit
AGCATGAGAAACCACCACAGTAGCTTTAGAATAGATGCGGTCATATTCTT6103010.10836481497987292No Hit
GTTGTCAAGATGCTACCGTTCAGAGTTACCCGCAGGAAGACGTATACCCC5965080.10591573511105838No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA5888220.10455101185493508No Hit
GCCTATCACCTCTTATGCACACCTCCCACCCCCTGTATTCCCACCCCTGG5819500.10333082213127137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)