FastQCFastQC Report
Mon 13 May 2024
EGAF00005475296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335193909
Total Bases25.8 Gbp
Sequences flagged as poor quality0
Sequence length77
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC31011060.9251677661004275No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA24248560.7234188733423553No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT15523450.46311849897009916No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGG14546090.4339604512324238No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT11125030.3318983341072585No Hit
TAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGG10486660.3128535369656732No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT10346040.3086583533354122No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT9425850.281205885516255No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC8166550.24363658708368713No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT7885350.235247413162272No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA6754140.2014994848847328No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT6559330.1956876250994167No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT6089180.18166141557184443No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT5826660.17382953101334547No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG5067540.15118234144284526No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA5037240.1502783870693784No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC5021380.14980522811349772No Hit
AGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATA4463210.13315307588122074No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG4226880.12610253010295602No Hit
TGACCCTTCAAGCTCTGTCCTTATCCATTACCTCAAAGCAGTCATTCCTT3951320.11788161699561193No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT3923860.11706238969873405No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC3717380.1109023732289837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)