FastQCFastQC Report
Thu 16 May 2024
EGAF00005475303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89878851
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCGAATGACTGGCCTGCGAAGGGCTGGTGGTAGCGGTGACAACAAGTG3770020.41945574048337575No Hit
TTGTTCCAAACTGGCCTGCGAAGATAGTTCGGTAGCGGTGACACATAACG2427450.27008022165303386No Hit
AACTGTATTACTGGCCTGCGAGACACTTAAGGTAGCGGTGACAACAGTAA1992020.2216338969442322No Hit
AACTGTATTACTGGCCTGCGAACTTGATGAGGTAGCGGTGACATCTCACG1693620.18843365053698785No Hit
TAGCTTGTAACTGGCCTGCGAGGAGTCTAAGGTAGCGGTGACACTAGATA1430810.15919317882690778No Hit
GCAATCCGAACTGGCCTGCGATAGTGACTAGGTAGCGGTGACAAAGGGAA1408900.15675545295967347No Hit
CAGAATCGTACTGGCCTGCGAAGTGAAAGGGGTAGCGGTGACAAACGTGT1299650.14460020188731607No Hit
AACCGCCTCACTGGCCTGCGACACCAGGTAGGTAGCGGTGACAATATTGT1215920.13528432845675786No Hit
TCTCTTCAAACTGGCCTGCGAATTACCAAGGGTAGCGGTGACAAGTAATT1166140.12974576188117937No Hit
ATGGATCCAACTGGCCTGCGAAAGCTACTTGGTAGCGGTGACAAACATCC1038190.11550993236440017No Hit
CTTCACATAACTGGCCTGCGAATCCAACCGGGTAGCGGTGACAGATCAGC981680.10922258007058858No Hit
ATTGGCTAAACTGGCCTGCGATTGGTATGAGGTAGCGGTGACAACTTACG914630.10176253810810287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)