FastQCFastQC Report
Thu 16 May 2024
EGAF00005475304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89878851
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT55288886.15148940878205No Hit
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC51868965.770986102169909No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT36868274.101996141450451No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA34681093.858648571286253No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC28346333.153837603019647No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC25119782.7948488126533793No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG21503552.392503882809984No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT13670661.5210096533165516No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA11193211.2453663876944754No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCTCCAATC10825981.2045080549594476No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG10821171.2039728901296258No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC8400740.9346737198498455No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT8055910.8963076308129484No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG7904450.8794560580219256No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT6992610.7780039377672952No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG6919600.7698807809636996No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA6286960.6994926982322015No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA5938300.6607004800272759No Hit
TGACCCTTCAAGCTCTGTCCTTATCCGTTACCTTAAAGCAGTCATTCCTT5783190.6434428050265129No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA5744610.6391503602999998No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA4896600.5448000219762489No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG3865820.4301145327280608No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC3857460.4291843917764369No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT3478420.3870120680559212No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT3311310.36841926250258805No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA3272460.3640967773386422No Hit
GCTGGCCATTATTAAGTCCCTGTAACTCAAATGTCAACCCCACCGAGGCA3184980.3543636756104058No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA3159160.3514909197047924No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT3069110.34147187751654723No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT2951140.3283464315759889No Hit
CCTGTGCTGGCGCTGGAAAATTATTTGTGCTCAGCTGACATTTAACACTC2614710.29091493392589096No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC2503680.2785616384882357No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT2397310.26672681874849513No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCT2373140.2640376432938601No Hit
GGTACGGGCCAGGAAGAATATGTGGCAGAGCTCCTGGAAATGATGCAGAT2371800.26388855371548975No Hit
CTCTGAACCCCAATCCTCAAAATGAAGATACTGACACCACCTTTGCCCTC2346210.26104138781213393No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG2340430.2603982999293126No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC2304990.25645521436405544No Hit
CTCTTCTACAGTACCACCGTCACCTTGTTCAAGGTGAAATGATCCCAACA2175560.24205471874579257No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC2124840.2364115669435961No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT2053820.22850981928996847No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG2047740.22783335314333292No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT2039830.22695327958743042No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG2030880.22595749471697185No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA2029510.22580506731222008No Hit
GCCTATCACCTCTTATGCACACCTCCCACCCCCTGTATTCCCACCCCTGG2002920.2228466405294834No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG1871420.2082158348909022No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGTTCCTGCCCTTG1832190.20385107059279162No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG1807410.2010940260017343No Hit
GGCCATGATTATCTTAAAGGCATTATTCTCCAGCCTTAAGATCTTAGGAC1803300.200636743787479No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG1776600.19766607830801042No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA1775640.19755926786380482No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1687950.18780280135089844No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT1665520.18530721982638607No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT1612860.17944822191819074No Hit
GTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTC1589610.17686140647258608No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT1423430.15837207353707716No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA1420810.15808057003309933No Hit
CCCGTCCTGGCTATGAAACCTTGTGAGCAGAAGGCAAGAGCGGCAAGATG1353730.1506171902442322No Hit
ATGGCCACAGTAGTCTTGATGACCAAAGTCCTTTTTTTCCATCTTTAGAA1343920.1495257210175061No Hit
GTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAA1337250.14878361095203588No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC1320610.1469322299191386No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT1308570.14559264893139323No Hit
TGGAGATGACCACGCCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGC1276370.14201004861532998No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT1239410.1378978465134139No Hit
GAGCAGTGAATCAAGGCAGACTTATGAAATCTGTATTATATTTGTAACAG1230150.1368675707703473No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG1154810.1284851761177944No Hit
TGGGCATTTGCTACATGATGAGTGCTGCCAGATTGTGGCAGGTAAAGAGA1151940.12816585739397138No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGGTGTGGCATGCAGCATTT1147860.12771191300609752No Hit
ACCTAAATTAAGACTCTGACTTGGATTGTGAATTATAATGATATGCCCCT1131940.12594063980635445No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT1097610.12212105381721002No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCGTCTGAA1084510.12066353629732093No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC1066260.11863302524862052No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC1034440.11509270406672199No Hit
ATATATTGGCAGCAACTCCGAGAGAAATGCTTGTTATTTTCTCACACTGT1009720.11234233512842748No Hit
GCAATATGTGATGTGGCAAATCTCTATTAGGAAATATTCTGTAATCTTCA1008220.11217544380935623No Hit
CCTGATGTGCATATCCGTACTTCCATGAGGTGTTTTCTGTGTGCAGAACA1005550.11187837726140935No Hit
TCAACGAGATCGAGACCATCCTGGCCAACATGGTGAAACCCTGTCTGTAC1002290.11151566679462781No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA964620.10732446946835135No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG959380.10674146246039572No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA950390.10574122715476192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)