FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41027028
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA375100.068.395051
ACCTTGC449750.067.7593841
GCTGCCA435850.067.735591
CGCTATA377900.067.717473
GCTTGTA350750.067.696523
CGGTCCA528350.067.643881
CTTGCGG449800.067.601083
CCCGGAG424300.067.573643
TCGCTAT378800.067.5006942
TTGTTCC322500.067.47231
CTGTCAA466250.067.436014
GTCCTTA388300.067.41433
TAAGTTC488200.067.39791
TACCGAG426050.067.366341
CCTTAAC791050.067.330285
ATGGATC418500.067.279131
TCCCGGA426100.067.2464752
AAACCGC475150.067.224542
CCTTACA410550.067.221942
TGGATCC415200.067.183722
Produced by FastQC (version 0.11.5)