FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005475306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41027028
Sequences flagged as poor quality0
Sequence length75
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCAT11894962.899298481966571No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATAAGTTAACTTCCAATTTACATACTCT4576341.1154451645875982No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAG3536600.862017107356643No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTTTGCAAAGGCCCTGGCCAACGTCAAC2403140.5857455724065609No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACATGCTGACTGTCCTCTCCCCTCCAGCC2043370.49805459951912673No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTGCCTTT2038380.4968383281382215No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCTTTCTAAATTGCTGTTCCTTAATTTG1630730.3974769997963294No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCTGGTGGGGCTGTAGCCGTCTCTGCTG1500450.3657223233425536No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTT1420700.346283918006442No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCT1328450.32379874067407466No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGAATGAGC1248020.30419459094136675No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAATGTGTCTGCGTCCCTGTTAGCCTAA1166240.2842613898330632No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCTATAGCCACCCCAAGTGTGGTTATGC1112030.2710481490397013No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAAGTTGCTAATATGCAGAAGGGATGGG1090270.26574432834861933No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGA1083040.26398207542598506No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATCATCTTTCCTGTTCCAGAGAGGTGGG1008590.24583550141628585No Hit
CAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGGTGGACCTCATGGCCCACATGGCCTC1008520.24581843949310683No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTATAATGCAGAGTGAGAACTTTCCCTAC821950.2003435393857922No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGGTTGGAAAGTCCAAGCCGTAGGACCC808310.19701890178347795No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAATGTGTCTGCGTTCCTGTTAGCATAA761900.18570684671577964No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAG752390.1833888625810283No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGGTGACGAGGGCCCTGGCCACCACCAT665310.16216383014631233No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCA660580.16101093162292918No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACGTTTTATTGTGTTTTTAATTTATTTA592020.14429999657786569No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTAAATTGGCATGGAAATTTAAAGCAGG584110.14237199925863508No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATCACGTAAAGCTAGAAATGATCCCCAG571290.13924722989927518No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAAACCTGTTAAATTTTTCTGGACAATG532220.12972423934777827No Hit
TAGGTGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAATACCTCAATTTTTGTTTTTGAGTACT513960.12527351481564786No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGATGAATGAGCGGTAGCTTTAGTTTGT490130.11946514868198593No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCCCCATTCCCTCTCTACTCTTGACAGC478400.11660605784069955No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATGTGCTGCTGATCTGGGCCTGAATAAG437440.10662239536336875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)