FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51872946
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA451950.068.3921
CGCTATA453750.068.008673
TACCGAG566200.067.784011
TCGCTAT454600.067.706162
TGGATCC559850.067.6543352
GCTGCCA563300.067.587861
ACCTTGC534250.067.5367661
TTGGCTA594400.067.5296252
CCCGGAG466300.067.480543
ATGGATC566050.067.442361
CTTGCGG536300.067.402853
CGGTCCA542500.067.342831
GCTTGTA420550.067.339343
CCTTAAC908100.067.333295
CCTGAGC628100.067.332851
AAACCGC609550.067.30592
TAAGTTC562100.067.30251
CCGCCAC600900.067.299725
GTCCTTA486300.067.294913
TACCTTA429300.067.220573
Produced by FastQC (version 0.11.5)